The Stellenbosch University Botanical Garden located in the historical center of Stellenbosch is the oldest university botanical garden in South Africa. The Garden is relatively small and houses an enormous diversity of plants, both indigenous to South Africa and introduced species. It is open to the public.

The history of the Stellenbosch University Botanical Garden dates back to 1902 when lecturer Augusta Vera Duthie grew plants next to the then Main Building on campus for research and student practicals. The Botanical Garden's current site dates back to 1922 when Gert Cornelius Nel, professor of botany at the time convinced the University Council to allocate land for the establishment of the Garden.